eCHO Systems

Scientific Background

Hi, my name is Linas and I’m from Lithuania, small country in the Baltic region. I have been immersed in the field of biochemistry since high school, having participated in chemistry Olympiads and having A levels in Biology, Chemistry and Physics. At Vilnius University I finished a bachelors and masters in biochemistry, working within the field of biofilm elipsometry in the Centre for Physical Sciences and Technology. While the field is far from CHO cell engineering it gave me a good starting point to start developing myself as a researcher, and I am grateful to my supervisors for the opportunity to work in a highly interdisciplinary background.

Linas Tamosaitis, UoK

Why have you chosen to do a PhD in an international collaboration project?

During my time in university I volunteered and attended several international conferences during which I realized that modern science is built around the backbone of collaborations. Being part of a team of multi-cultural, diverse and talented people is also one of the greatest opportunities for personal and professional growth. Therefore, applying for a Marie-Curie ITN seemed like the perfect match.

My lab and the PhD-project I am doing

Recently, CHO cell research is experiencing a paradigm shift towards synthetic and systems biology due to the availability of a variety of omics data. The Chinese hamster, CHO K1 has been sequenced and published along with a library of histomic, proteomic, transcriptomic data. I am trying to employ siRNA high-throughput screening which is a widely used technique for discovering the relationship between gene and phenotype. My screen will be using a list of genes identifi­ed as commonly differentially expressed in CHO with good growth, qP and also pathways that are enriched from the assembled list of unique genes in literature. Using this method we aim to identify the key genes associated with a high producing phenotype

I am based in the lab of prof. Mark Smales in the University of Kent, UK. His group in particular focusses upon the investigation of cultured mammalian cells for the purposes of producing biotherapeutic proteins for the treatment of disease and for the generation of Diagnostics.

What kind of tips would you give to future PhDs taking part in an ITN?

One of the biggest challenges for a person in an ITN can be the culture shock if you haven’t travelled much. Be aware of the differences between cultures, be flexible and open minded.  Never be afraid to ask question or for help when dealing with something outside of your expertise. We are all here to learn. Most importantly, always try to maintain quality in not just your professional, but in your personal environment.