eCHO Systems

Dr Helene Faustrup Kildegaard

About the Lab
The CHO Cell Line Engineering and Design Section is addressing the need to rapidly obtain high yields and high quality of biopharmaceuticals produced in optimized CHO cells through genome-scale-based methodologies. Our main goal is to develop state-of-the-art genome editing technologies, omics data generation and high-throughput screening methods to improve CHO cell engineering efforts and facilitate identification of factors, that control growth, protein production and processing. The section is part of The Novo Nordisk Foundation Center of Biosustainability at the Technical University of Denmark. Currently we host 4 post docs, 7 PhD students and 1 technician in our group.

eCHO project 
CRISPR-mediated genome editing tools are valuable for accelerated and targeted engineering of CHO cell factories. This project focuses on improving the editing tools to facilitate multiplexing and development of a flexible donor DNA construction platform for targeted integration. In addition, the project focuses on developing a high-throughput target-screening platform.

Research interest

My research focuses on applying ‘omics data, high throughput technologies and efficient genome editing tools to engineer CHO cell factories for increased production and improved quality of therapeutic proteins. I have a special interest in genome engineering and my main goal for the future is to enable production of novel therapeutics through cell line Engineering.