Prashant Prashant, DCU
Why have you chosen to do a PhD in an international collaboration project?
My Master thesis experience at NICB was tremendously valuable for acquiring/developing scientific and interpersonal skills. This first international exposure turned out to be a continuous learning process.
My lab and the PhD-project I am doing
Project title: “Proteomics and post-translational regulatory networks”
At the proteomic facility of the National Institute for Cellular Biotechnology, Dublin City University, Dublin, Ireland my primary objective is to identify phosphoproteins using enrichment techniques prior to MS analyses and applying these methods to a wide range of CHO cells during bioprocess related conditions. Moreover, site specific phosphorylation of CHO proteins will be assessed by mass spectrometry.
Key proteins identified will also be analysed for their phenotypic effect on cells using siRNA knockdown and cDNA overexpression approaches.
What kind of tips would you give to future PhDs taking part in an ITN?
My “secret” tip for future candidates would be to enjoy this exceptional PhD program with a learning attitude. Having an open-minded approach toward different societies and cultures is a prerequisite. Living in Ireland and traveling to various European destinations has helped me learn a lot. Before joining this programme, I thought Wiener Apfelstrudel is an Austrian town - now it’s my favourite desert for example.
I obtained my Bachelor’s degree in Engineering followed by a Master of Technology, with a major in Biotechnology from the National Institute of Technology in Bhopal, India. My scientific interest has always revolved around proteomics, especially in learning fundamentals of protein chemistry and understanding cell signalling processes. With this regard I was fortunate to work at the Proteomics facility of the National Institute for Cellular Biotechnology at Dublin City University, Dublin, Ireland for my master’s thesis. At NICB I conducted proteomic analyses on colorectal cancer cell lines for protein biomarker identification with a special focus on the 14-3-3 family of regulatory proteins.